First molecular detection of <i>Brachyspira suanatina</i> on pig farms in Poland

Abstract Introduction Prior to the 2000s, swine dysentery was considered be caused only by Brachyspira hyodysenteriae with contributing commensal intestinal anaerobes. Nowadays, it is known that disease three strongly beta-haemolytic species of anaerobic spirochaetal genus Brachyspira, i.e. B. and newly emerged hampsonii suanatina. Material Methods The present investigation carried out in November 2022 on nine Polish high-performing finisher pig farms. At every location one fresh pooled faecal sample collected from 40 randomly selected pigs between 60 110 kg live weight. Nucleic acid extracted each analysed an in-house multiplex PCR for spp . , which capable confirming detecting differentiating species. Results From a total samples examined, genetic material suanatina detected seven. Non-pathogenic/questionably pathogenic spp. were found six samples. Conclusion To best our knowledge, this first report identification outside Scandinavia, Germany United Kingdom. Our research not provides valuable epidemiological data infection Europe but also highlights both importance modern laboratory diagnostics need thorough across regions, including retrospective studies.